Voronoi cell-based model of the corneal epithelium: Dynamical model of self-renewal and wound healing
The corneal epithelium is a transparent, self-renewing tissue on the outer surface of the eye, which is maintained with remarkable precision. It is a stratified epithelium consisting of several layers, each only one cell thick. Maintaining this structure is essential for high-quality vision.
Its regeneration is driven by limbal epithelial stem cells (LESCs), which reside on a ring around the bottom layer of the corneal epithelium. LESCs proliferate and produce transit amplifying cells (TACs) that migrate centripetally toward the centre. TACs continually replenish the tissue and, together with vertical migration, called delamination, between layers, they sustain the entire epithelium. Despite this highly coordinated renewal process, the mechanical mechanisms regulating epithelial stratification remain poorly understood.
We develop a Voronoi cell-based model (VCBM) of cell interaction and migration on the bottom two layers of the corneal surface. Our simulations reveal how intercellular interactions coordinate cell size, migration, and turnover to maintain and rapidly restore epithelial integrity. We then introduce an extension of the model to a five-layer structure and use it to study wound healing dynamics. Finally, we discuss preliminary formulations of our model as systems of partial differential equations.